Nuclear run-on transcription of A549 cells compared with HepG2 cells after induction with proinflammatory mediators. Newly synthesized transcripts were labeled with ³²P-CTP, the RNA isolated and used to probe denatured target DNA corresponding to FBG Aα, Bβ, and γ chain cDNAs. The autoradiograph of the slot-blots (A) were subjected to densitometric scanning and the tracings of control (CON) and treated (DEX + IL-6) A549 (B) and HepG2 (C) cells are shown. Plasmid pGEM3Z DNA served as negative control (Neg Con), and hybridization to human γ-actin cDNA was used to normalize the data between samples.