![Fig. 2. Dose- and time-dependent induction of Stat 1α protein and Stat1 mRNA by ATRA in NB4 cells. (A) NB4 cells (4 × 105/mL) were treated for 4 days with medium alone (medium) and medium containing the indicated concentrations of ATRA. Cells were harvested, lysed and the cell extracts (10 μg of protein/lane) subjected to Western blot analysis, using antibodies specific for Stat 1α. (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]). A Western blot representative of two independent experiments is shown. (B) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Western blot analysis was conducted as described in (A). The apparent molecular weight of standard proteins (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]) is indicated on the right. A Western blot representative of three independent experiments is shown. (C) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Total RNA was isolated and subjected (20 μg/lane) to Northern blot analysis. Filters were sequentially hybridized with cDNA probes coding for Stat 1α and β-actin. A Northern blot representative of two independent experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/3/10.1182_blood.v89.3.1001/8/bl_0032f2.jpeg?Expires=1735497255&Signature=UmGEKlaJgRZjcaGcwDIGyhdwldBdkRvlnNmgRNbWaWpNvE5qAoQN~sxPnpI~6q68kD8UKr7pvzn6r9FQgNaQ5Cb-5NtacGNoyS73mRjuVJSEhlRzFz8S29oG-UtMza3yU1Nx8MPqaFbnroAA7XnOt-2UfervGMTdhYiNqQJgs-jsVlnXhPFlyeTBzSn94qD4WQ3NyMtYOhv0xS4473pBFh-t45bBMNnEp3UCD51~dZvh9u5ebMFNzY9kBtxP2CrnB8JejVBQZJDPvUl0F0tnWVDVczi2ZQUkIK09QQ1ZbGeZtjetPvM-gLnsfYy7bBk5lDO3WzRgSjlIYNcKUBjx8Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dose- and time-dependent induction of Stat 1α protein and Stat1 mRNA by ATRA in NB4 cells. (A) NB4 cells (4 × 105/mL) were treated for 4 days with medium alone (medium) and medium containing the indicated concentrations of ATRA. Cells were harvested, lysed and the cell extracts (10 μg of protein/lane) subjected to Western blot analysis, using antibodies specific for Stat 1α. (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]). A Western blot representative of two independent experiments is shown. (B) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Western blot analysis was conducted as described in (A). The apparent molecular weight of standard proteins (carbonic anhydrase [28 kD], ovalbumin [43 kD], bovine serum albumin [69 kD], phosphorylase B [105 kD], and myosin heavy chain [215 kD]) is indicated on the right. A Western blot representative of three independent experiments is shown. (C) NB4 cells (4 × 105/mL) were treated for the indicated lengths of time with 10−6 mol/L ATRA. Total RNA was isolated and subjected (20 μg/lane) to Northern blot analysis. Filters were sequentially hybridized with cDNA probes coding for Stat 1α and β-actin. A Northern blot representative of two independent experiments is shown.
