Fig. 4.
![Fig. 4. Effect of HLE and Cat G on EC activation induced by thrombin or TRAP42-55. (A) [Ca2+]i was determined in fura 2-loaded IVECs in suspension preincubated for 5 minutes with 600 nmol/L Cat G or HLE before stimulation with 1 nmol/L thrombin () or 12.5 μmol/L TRAP42-55 (▨). (B) PGI2 synthesis was measured from supernatants of confluent HUVEC monolayers preincubated for 30 minutes with 50 nmol/L HLE or Cat G before stimulation with a similar concentration of the same agonists. Data are expressed as the percentage of the control values (no preincubation with HLE or Cat G). Each histogram is the mean ± SEM of three to four distinct experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.1944/3/bl_0019f4.jpeg?Expires=1735519008&Signature=oh1qzwgCYPpaOQNlhdTiLCmXhuXsQorQ3tVsudtMncFaE~F~9a-hixdJ~b7fI66KsfD2ehxXlBb2AVzNT3S4VWgD9cA5jvYVjnx3Qy3GIPLkfDwoF7noqA9qfG~Khr36Hye9O7NlVnIOvPhy27UjBubnWGHnCp6WFq8HnsDvhf-dXY3jWfJQ7tN4grTFcaM57uv~ghGsm7sp82QM7lNHlCB45zEaXgrYTLzk7FtLpyeRARmBJAbxVdUuNV9M1V7pZnXEIslRycN93pmqRm4RcsGsmJ5DIt~R2v3Rp29xO3Ung-X5kYE6SViR0~yKeYn5y8mFainQfXZcDqD~qGpOHw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of HLE and Cat G on EC activation induced by thrombin or TRAP42-55. (A) [Ca2+]i was determined in fura 2-loaded IVECs in suspension preincubated for 5 minutes with 600 nmol/L Cat G or HLE before stimulation with 1 nmol/L thrombin () or 12.5 μmol/L TRAP42-55 (▨). (B) PGI2 synthesis was measured from supernatants of confluent HUVEC monolayers preincubated for 30 minutes with 50 nmol/L HLE or Cat G before stimulation with a similar concentration of the same agonists. Data are expressed as the percentage of the control values (no preincubation with HLE or Cat G). Each histogram is the mean ± SEM of three to four distinct experiments.
