Fig. 6.
![Fig. 6. Effect of HLE, Cat G, and PR3 on platelet activation induced by thrombin or TRAP42-55. Stirred fura 2-loaded platelets were preincubated with buffer (A) or 600 nmol/L of each of the neutrophil proteinases (B to D). The preincubation time was 5 minutes except for Cat G (C), for which a longer period was required to allow the Ca2+ mobilization induced by this proteinase to return to the basal value. After stimulation of platelets with 5 nmol/L thrombin, platelets were further challenged with 6.25 μmol/L TRAP42-55. Changes in fluorescence intensity reflect changes in [Ca2+]i and are representative of three distinct experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/6/10.1182_blood.v89.6.1944/3/bl_0019f6.jpeg?Expires=1735519050&Signature=NaBuSRhKYlCnWR0oNOPjvMtNXkJPYMrHg9yBWSwG9a61w6JaG8DiDRDF64kpdhoZWc2WKlkygMA2Xz~IAV1wpPPIb3koP33q7wm0N8V05JorPr~yalJQUd71ZLCfby2pBQur04mI~p-G8fFwwLc2KfgcS43l7NsS-b2y-rkUiwf7uX~RMnCmpedHdMDbT3DbgyocHG4kcqt~pACHJFJLVW7CPEJrzceFTHt8a8os9aPkaoGlAS~xvOxd2xEjKO1GERiQf1hrqw5pog4ZUFsdCZJ0DSmF8aTXw0yWgN4w6EHiHI3J0CeEUVPMVSQJY4xdTyAcuYzArCJ3X50eTE6Zbw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of HLE, Cat G, and PR3 on platelet activation induced by thrombin or TRAP42-55. Stirred fura 2-loaded platelets were preincubated with buffer (A) or 600 nmol/L of each of the neutrophil proteinases (B to D). The preincubation time was 5 minutes except for Cat G (C), for which a longer period was required to allow the Ca2+ mobilization induced by this proteinase to return to the basal value. After stimulation of platelets with 5 nmol/L thrombin, platelets were further challenged with 6.25 μmol/L TRAP42-55. Changes in fluorescence intensity reflect changes in [Ca2+]i and are representative of three distinct experiments.
