Fig. 1.
Kinetics of DNA synthesis by GC and resting B cells. B cells were cultured for 10 days in the presence of CD40 MoAb, G28-5 (•), PMA with ionomycin (▵), or CM alone (○). [3H]thymidine uptake was measured after the intervals shown. (A) GC B cells; (B) GC B cells in the presence of CD32-L cells; (C) resting B cells in the presence of CD32-L cells. Data are mean cpm for cells purified from the same tonsil and representative of five experiments. Values for [3H]thymidine uptake by CD32-L cells alone (∼4,000 to 10,000 cpm) have been subtracted in (B) and (C).