Fig. 5.
Fig. 5. Kinetics of DNA synthesis by GC and resting B cells cultured with combinations of cytokines. GC B cells (A and B) or resting B cells (C and D) were cultured for 10 days with CD32-L cells in the presence (A and C) or absence (B and D) of CD40 MoAb, G28-5, and various cytokine combinations or CM (○). [3H]thymidine uptake was measured after the intervals shown. (□) IL-10+IL-1β+IL-3; (▴) IL-10+IL-1β+IL-2; (•) IL-10+IL-7+IL-4; (▵) IL-10+IL-3+IL-6. The combination of IL-10+IL-7+IL-3 gave a similar profile to that of IL-10+IL-3+IL-6. Data are mean cpm for cells purified from the same tonsil and representative of four experiments.

Kinetics of DNA synthesis by GC and resting B cells cultured with combinations of cytokines. GC B cells (A and B) or resting B cells (C and D) were cultured for 10 days with CD32-L cells in the presence (A and C) or absence (B and D) of CD40 MoAb, G28-5, and various cytokine combinations or CM (○). [3H]thymidine uptake was measured after the intervals shown. (□) IL-10+IL-1β+IL-3; (▴) IL-10+IL-1β+IL-2; (•) IL-10+IL-7+IL-4; (▵) IL-10+IL-3+IL-6. The combination of IL-10+IL-7+IL-3 gave a similar profile to that of IL-10+IL-3+IL-6. Data are mean cpm for cells purified from the same tonsil and representative of four experiments.

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