American Society of Hematology

Fig. 4.

$Fig. 4. (A) The effect of the concentration of (•) DFO, (○) PIH, and the PIH analogues (▾) 206, (♦) 308, (⬡) 309, (▪) 311, and (▵) 315 on 59Fe release from prelabeled SK-N-MC NB cells. (B) The distribution of 59Fe between the efflux medium, stromal-mitochondrial membrane (membrane), and cytosol after incubation of prelabeled SK-N-MC NB cells with DFO and analogue 311. In these experiments, SK-N-MC cells were prelabeled with 59Fe-Tf (1.25 μmol/L) for 3 hours, washed, and then reincubated with either medium alone or medium containing the chelators (2 to 50 μmol/L) for 3 hours at 37°C. Cells were disrupted and the cytosolic and membrane fractions were prepared as described in the Materials and Methods. Results are means of triplicate determinations from a typical experiment.$

(A) The effect of the concentration of (•) DFO, (○) PIH, and the PIH analogues (▾) 206, (♦) 308, (⬡) 309, (▪) 311, and (▵) 315 on ⁵⁹Fe release from prelabeled SK-N-MC NB cells. (B) The distribution of ⁵⁹Fe between the efflux medium, stromal-mitochondrial membrane (membrane), and cytosol after incubation of prelabeled SK-N-MC NB cells with DFO and analogue 311. In these experiments, SK-N-MC cells were prelabeled with ⁵⁹Fe-Tf (1.25 μmol/L) for 3 hours, washed, and then reincubated with either medium alone or medium containing the chelators (2 to 50 μmol/L) for 3 hours at 37°C. Cells were disrupted and the cytosolic and membrane fractions were prepared as described in the Materials and Methods. Results are means of triplicate determinations from a typical experiment.

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