Detection of the rAAV-encoded transcript in erythroleukemia (K562) cell colonies. Erythroleukemia cells were transduced with rHS432^Aγ* at an MOI of 3 × 10⁸; cultured in semisolid medium for 14 days, after which individual colonies were plucked; and expanded for 2 to 4 days in liquid medium. An aliquot of the cells was used for RNA extraction. Lanes 1 through 12, RT-PCR analysis to detect the ^Aγ* transcript; (+) is with reverse transcriptase and (−) is the control without reverse transcriptase. Lane 13, water blank for the PCR reaction. Lane 14, RNA extracted from a cell line containing a single integrated copy of the ^Aγ* gene linked to HS2. Lane 15, RNA from control K562 cells that had not been transduced.