Western blot analysis of the hMLH1 protein. hMLH1 protein is observed around 85 kD. The lower bands detected around 70 kD shows expression of Hsp70, as controls for the amounts and integrity of protein. Lane 1 represents normal lymphocytes derived from a normal volunteer used as a normal control. Lanes 2 through 7 are cell lysates prepared from cell lines MOLT16 (lane 2), P30OHK (lane 3), CEM (lane 4), KCL22 (lane 5), BALL1 (lane 6), and U937 (lane 7). P30OHK, CEM, and KCL22 had loss of hMLH1 protein expression with normal expression levels of Hsp70.