Fig. 2.
Kinetics of the priming effect of EBV on the synthesis of LTB4 and LTC4 . Enriched monocyte suspensions (10 × 106 cells/0.1 mL) were preincubated or not with EBV for various periods of time at 37°C, diluted 10-fold with HBSS, and stimulated for 5 minutes with 75 nmol/L A23187. Levels of LTB4 (A) and LTC4 (B) in incubation media were measured by RP-HPLC as described in the Materials and Methods. Values are the mean ± SD of triplicate incubations from one experiment representative of five separate experiments.