American Society of Hematology

Fig. 4.

$Fig. 4. Effect of EBV on the release of arachidonic acid in fMLP-stimulated enriched monocyte suspensions. Enriched monocyte suspensions (2 × 106 cells/0.1 mL) obtained by centrifugation on a Percoll density gradient were incubated or not with EBV (for 20 minutes) at 37°C, diluted 10-fold with HBSS, and stimulated with fMLP (1 μmol/L for 10 minutes). Samples were subjected to RP-HPLC and fractions containing arachidonic acid were collected for quantitation by mass spectrometry as described in the Materials and Methods. Results (mean ± SD) are from one experiment representative of three separate experiments performed in triplicate.$

Effect of EBV on the release of arachidonic acid in fMLP-stimulated enriched monocyte suspensions. Enriched monocyte suspensions (2 × 10⁶ cells/0.1 mL) obtained by centrifugation on a Percoll density gradient were incubated or not with EBV (for 20 minutes) at 37°C, diluted 10-fold with HBSS, and stimulated with fMLP (1 μmol/L for 10 minutes). Samples were subjected to RP-HPLC and fractions containing arachidonic acid were collected for quantitation by mass spectrometry as described in the Materials and Methods. Results (mean ± SD) are from one experiment representative of three separate experiments performed in triplicate.

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