Effect of TFK-AA on LTB₄ synthesis in EBV-primed PBMC. PBMC (10 × 10⁶ cells/0.1 mL) were preincubated (or not) with EBV for 15 minutes at 37°C, diluted 10-fold with HBSS, and stimulated with A23187 (50 nmol/L for 10 minutes) in presence of increasing concentrations of the cPLA₂ inhibitor AACOCF3 added to the cell suspensions 5 minutes before A23187. Results (mean ± SD) are from one experiment representative of three experiments performed in triplicate.