Heteroduplex analysis of the indicated TCRBV chains PCR products of different T-lymphocyte subsets prepared from the RA patients ROPI, GEGU, LOME, and MAGHI. PCR products obtained from PCR amplification of the cDNA of the J77 and C1-632 cells27 were used as mono and polyclonal controls. ++, CD4⁺CD57⁺ T-cell subset; +− CD4⁺CD57⁻ T-cell subset; −+, CD4⁻CD57⁺ T-cell subset; and − −, CD4⁻CD57⁻ T-cell subset.