Multiplex RHD PCR of genomic DNA derived from D^VI phenotype individuals. gDNA derived from peripheral blood lymphocytes isolated from individuals expressing D^VICcee and D^VIccEe phenotypes were analyzed using a RHD intron 4/exon 10 multiplex assay (see Materials and Methods). Six different individuals with the D^VI phenotype are illustrated (donor identification in brackets). A D-positive (DCe), D-negative, and no template controls are also shown. PCR products were analyzed by electrophoresis on 6% polyacrylamide gels.