Fig. 5.
Fig. 5. Fas− cells express FasL after strong cross-linking of HLA class II molecules. Fas− cells were incubated for 96 hours with (a) L227 XL, (b) L227, (c) goat antimouse F(ab′)2 , or (d) W6.32 XL. The level of expression of nonstimulated cells is also shown as an unfilled profile. FACS profiles were obtained according to the method used in Fig 1a to d. (e) FasL detection after SDS-PAGE in nondenaturing conditions as a 110-kD trimer in lane ii. Lane 1, nonstimulated; lane 2, L227 XL; lane 3, W6.32 XL.

Fas− cells express FasL after strong cross-linking of HLA class II molecules. Fas− cells were incubated for 96 hours with (a) L227 XL, (b) L227, (c) goat antimouse F(ab′)2 , or (d) W6.32 XL. The level of expression of nonstimulated cells is also shown as an unfilled profile. FACS profiles were obtained according to the method used in Fig 1a to d. (e) FasL detection after SDS-PAGE in nondenaturing conditions as a 110-kD trimer in lane ii. Lane 1, nonstimulated; lane 2, L227 XL; lane 3, W6.32 XL.

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