Effect of GC on the growth of (A) KS cells, (B) HUVEC, and (C) AOSM and KS cell cycle (D and E). KS cells (KSC-2), HUVEC, and aortic smooth muscle cells (AOSM) were each assayed by plating the cells in 24-well plates and treating with hydrocortisone (HC) on days 1 and 3, and the cells were counted on day 6. The assays were done in triplicate; results are shown as mean ± SE. KS cell cycle analysis (D and E): Serum-starved KS cells were cultured in T-75 flasks without (D) or with (E) HC (10−6 mol/L) for 24 hours. Cells were harvested, DNA stained with propidium iodide, and cell-cycle was analyzed by flow cytometry. The results were expressed as DNA content in the different phases of the cell cycle. Channels 68-86 represent cells in G1 phase, channels 86-138 represent cells in S phase, and channels 138-160 represent cells in G2 + M phase.
