GCs downregulate (A) IL-1 and IL-6, (B) IL-6 protein, (C) bFGF, and (D) VEGF. For mRNA studies, KS cells were seeded in 75-cm2 flasks and treated with hydrocortisone (10⁻⁶ and 10⁻⁷ mol/L). The cells were obtained at 6 or 24 hours. Total RNA was extracted and assayed by the Northern method. The membranes were also probed for β-actin to normalize for the quantity of RNA. For IL-6 protein assays, equal numbers of cells were seeded in triplicate wells and treated with hydrocortisone (10⁻⁶ mol/L). Supernatants were collected at various time points, cleared of cell debris by centrifugation, and assayed by ELISA for IL-6. The results represent mean ± SE.