Effect of GC on the levels of (A) tPA, (B) PAI, and (C) uPAR. Equal number of KS cells were seeded in 75-cm2 flasks and treated with HC (10⁻⁶ mol/L). The supernatants were collected at different time points and tPA and PAI-1 levels were measured by ELISA. For the uPAR assay, protein extracts were quantitated by the Bradford method and the levels of uPAR were measured by ELISA and normalized for the total protein in each sample. The results represent the mean ± SE of triplicate experiments.