Reverse zymography for TIMP-1. A total of 15 μL of conditioned media was electrophoresed on 15% SDS polyacrylamide gels containing gelatin and HT1080 fibroblast culture-conditioned media. SDS was removed following electrophoresis and gels incubated to restore gelatinase activity. Gels were scanned by an AGFA Arcus flatbed scanner. Lane A, HT1080-conditioned media (positive control containing both TIMP-1 and TIMP-2); lane B, AG876; lane C, Jijoye; lane D, PA682; lane E, DW6.