TIMP-1 and TIMP-2 protein expression. (A) Molt-4 cytocentrifuge preparations stained with rabbit polyclonal anti-TIMP–1 followed by FITC-conjugated goat antirabbit antibodies. Excitation wavelength 490 nm. Original magnification × 630. Small intensely fluorescent structures are debris (too small for cells and no nuclear content on light microscopy). (B) K562 cytocentrifuge preparations stained with rabbit polyclonal anti-TIMP–1 followed by FITC-conjugated goat antirabbit antibodies. Excitation wavelenght 490 nm. Original magnification × 630. (C) Flow cytometric analysis of TIMP-2 expression in K562 cells. Cell number represented on Y-axis and fluorescence intensity on X-axis. Filled in histogram staining with nonimmunized rabbit serum (negative control), clear histogram staining with TIMP-2. Histograms overlap. (D) Flow cytometric analysis of TIMP-2 expression in Jurkat cells. Cell number represented on Y-axis and fluorescence intensity on X-axis. Filled in histogram staining with nonimmunized rabbit serum (negative control), clear histogram staining with TIMP-2.