Gelatin zymography for Gelatinase B. A total of 16 μL of conditioned media was electrophoresed on 10% SDS polyacrylamide gels containing gelatin. SDS was removed following electrophoresis and gels incubated to restore gelatinase activity. Gels were scanned by an AGFA Arcus flatbed scanner. Lane A, Gelatinase A; lane B, SUDHL-6; lane C, Molt-4; lane D, Jijoye; lane E, PA682; lane F, tonsil; lane G, HT1080-conditioned media (positive control for Gelatinase A and Gelatinase B.