(A) Reverse zymography of peripheral blood T cells. A total of 15 μL of conditioned media was electrophoresed on 15% SDS polyacrylamide gels containing gelatin and HT1080 fibroblast culture-conditioned media. SDS was removed following electrophoresis and gels incubated to restore gelatinase activity. Gels were scanned by an AGFA Arcus flatbed scanner. Lane A, HT1080 cell-conditioned media (positive control for both TIMP-1 and TIMP-2); lane B, unstimulated peripheral blood T-cell–conditioned media; lane C, IL-2/PHA-stimulated peripheral blood T-cell–conditioned media. (B) Gelatin zymography of peripheral blood T cells. A total of 16 μL of conditioned media was electrophoresed on 10% SDS polyacrylamide gels containing gelatin. SDS was removed following electrophoresis and gels incubated to restore gelatinase activity. Gels were scanned by an AGFA Arcus flatbed scanner. Lane A, HT1080 cell-conditioned media (positive control for both TIMP-1 and TIMP-2); lane B, unstimulated peripheral blood T-cell–conditioned media; lane C, IL-2/PHA-stimulated peripheral blood T-cell–conditioned media.