Soluble Fn does not inhibit dgRTA-induced permeability. HUV-EC-C monolayer cultures and permeability measurements were performed as in Fig 3, except that medium containing 5% fetal bovine serum was used. Monolayers were untreated (n = 2; ○), treated with 100 μg/mL soluble Fn (n = 2; □), or exposed to 5 × 10⁻⁸ mol/L dgRTA with (n = 4; ▪) or without (n = 4; •) 100 μg/mL soluble Fn. (A) Permeability is expressed as the mean fluid flux ± standard deviation. (B) Data from (A) for Fn + dgRTA- and dgRTA-treated monolayers expressed as the fold change in fluid flux relative to Fn-treated and untreated control monolayers, respectively.