Total cellular RNA was isolated by the acid-guanidium thiocyanate-phenol-chloroform method from Jurkat, Hut102, normal PBMC, normal PHA-blasts, and from PBMC or LN cells of five ATL cases. Single-strand cDNA synthesis and subsequent PCR reaction was done as described in Materials and Methods. PCR products were electrophoresed through 1.5% agarose gel, stained with ethidium bromide, and visualized on a UV-transilluminator. On the right, dilutions indicate the ratios of Hut102 cells to Jurkat cells.