American Society of Hematology

Fig. 5.

$Fig. 5. Migration of CBMC to C3a (A) and C5a (B). CBMC were obtained from the mononuclear cell fraction of umbilical cord blood cultured for 5 to 7 weeks in RPMI 1640 supplemented with SCF (100 ng/mL) and IL-6 (70 ng/mL). Migration of CBMC to C3a (A) and C5a (B) was assayed in Boyden chambers. The mean ± SEM of the spontaneous migration of CBMC to medium/1% BSA was 13.1 ± 3.3 cells/high power field and was referred to as 100% migration. Data represent the mean ± SEM of two experiments performed in triplicate (total n = 6).$

Migration of CBMC to C3a (A) and C5a (B). CBMC were obtained from the mononuclear cell fraction of umbilical cord blood cultured for 5 to 7 weeks in RPMI 1640 supplemented with SCF (100 ng/mL) and IL-6 (70 ng/mL). Migration of CBMC to C3a (A) and C5a (B) was assayed in Boyden chambers. The mean ± SEM of the spontaneous migration of CBMC to medium/1% BSA was 13.1 ± 3.3 cells/high power field and was referred to as 100% migration. Data represent the mean ± SEM of two experiments performed in triplicate (total n = 6).

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