Effects of a single intravenous injection of rmMGDF on megakaryocyte ploidy distributions in mice treated with 25 μg/kg () or 250 μg/kg (▪) MGDF. Megakaryocyte ploidy distributions were significantly different from the control distribution from 12 hours to day 9 in mice treated with 25 μg/kg of MGDF, and from 12 hours to day 10 in mice treated with 250 μg/kg of MGDF. From 387 to 1,136 (median 871) 4A5 positive cells with DNA contents ≥ 8N were analyzed per mouse. Each distribution shows the mean ± SEM of each megakaryocyte ploidy class; for the 25-μg/kg dose, n = 4-10 mice per MGDF group and n = 13 for controls; for the 250-μg/kg dose, n = 5-9 mice per MGDF group and n = 20 for controls. Controls received 0.1 mL/10 g body weight of 1% NMS in PBS.