H₂O₂ -accelerated apoptosis of CGD neutrophils. Neutrophils from normal donors (A) and CGD patients (B) were cultured with various concentrations of H₂O₂ in the presence (▪) or absence (□) of 100 ng/mL anti-CD95 MoAb. After 15 hours in culture, apoptosis of senescent neutrophils was estimated by flow cytometry described in Materials and Methods. (A) and (B) are representative of three independent experiments. (C) Appearance of fragmented DNA in CGD neutrophils incubated with H₂O₂ . The nuclear DNA samples extracted from CGD neutrophils cultured for 9 hours with medium alone (lane 1), 100 ng/mL of anti-Fas MoAb (lane 2), 1 mmol/L H₂O₂ (lane 3), or H₂O₂ plus anti-Fas MoAb (lane 4) were electrophoresed through 1.7 % of agarose gel as described in Materials and Methods.