Effect of IL-7 and IL-2 on the activation/expansion of anti-HIV CTL after Ag-IVS with vCP300. The CTL assay was performed at day 10 after IVS and data are reported as the percentage of specific lysis against vaccinia control (vP1170) and Gag/Pol-expressing (vP1206) autologous BLCL. The effector:target ratio was 10:1. Effector cells were depleted for CD4 or CD8 cells using magnetic beads as described in the Materials and Methods. (□) vCP300; (▨) vCP300 + IL-7; (▦) vCP300 + IL-2; (▪) vCP300 + IL-7 + IL-2.