Fig. 3.
Effect of fMLP on PMN adhesion under static conditions. Neutrophil adhesion to three different fibrin-coated surfaces was measured under static conditions: pure fibrinogen (▴), pure fibrin (○), and plasma fibrin (•). PMNs were incubated on the surface for 45 minutes at 37°C (2 × 106 cells/mL HEPES buffer) with or without fMLP. Shortly before measuring the total number of adhering PMNs per square millimeter, the surfaces were carefully rinsed to remove nonadherent cells. The data represent the number of adhering neutrophils per square millimeter. The mean values ± SE of six to eight experiments are given. *Significant effect of fMLP activation was determined by repeated measures ANOVA (P < .01).