Temperature-dependent degradation of ¹²⁵I-rhTPO by human PRP. PRP was incubated with ¹²⁵I-rhTPO at 0°C, 10°C, 20°C, or 37°C for 60 minutes. Platelet-associated radioactivity (n = 3 per data point) was then analyzed by SDS-PAGE and autoradiography. Nonincubated ¹²⁵I-rhTPO dosing solution (DS) was also run in the first lane of the gel. Temperature (in degrees Celsius) is indicated on the top of the figure and approximate MW (determined from prestained MW markers) is presented on the right axis.