Fig. 7.
![Fig. 7. Effects of protease inhibitors on the binding and degradation of 125I-rhTPO by intact platelets. Human PRP was incubated with 125 I-rhTPO with or without cold rhTPO, calpain inhibitor I [Cal (−)], or iodoacetic acid (Iodo) for 60 minutes at 37°C. Binding and degradation of 125I-rhTPO was determined (n = 3 per experiment) as previously described. *Indicates significantly less (P < .05) than total binding (TB), as analyzed by ANOVA followed by a Tukey-Kramer test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2782/4/bl_0002f7.jpeg?Expires=1735545783&Signature=p9-7WJHMEyrHsa~ChoHshu1hQ517ve5aDrc2ZjqvARytyRQYJXNCAR3B1edcRu3QtAR6cp2X-YIWxOj3RYvgpngCk05vacdFkGhqGP1AJ2cOqYx6a18WQNT-7CLh4CV3e5Th0TGSMQiAKswQhdJqBIVNtnO57zLbROgIFDlKe6RWr1y6ILTDfiitb3LSo3SivsUYdikf96bjXuSNrux1ZP-Clmb9cFVWp3B8d75e8uTWxsIZDaIw8p9dYr4ueSAfpsAl7~Dl2Sl4qNZWSmjDZnVh3BKnf50vGPqOQS~Ooa0LF9daS8FgcP1VYkMXWs4NxPanYYWrJiS3O099XKnVwQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of protease inhibitors on the binding and degradation of 125I-rhTPO by intact platelets. Human PRP was incubated with 125 I-rhTPO with or without cold rhTPO, calpain inhibitor I [Cal (−)], or iodoacetic acid (Iodo) for 60 minutes at 37°C. Binding and degradation of 125I-rhTPO was determined (n = 3 per experiment) as previously described. *Indicates significantly less (P < .05) than total binding (TB), as analyzed by ANOVA followed by a Tukey-Kramer test.
