American Society of Hematology

Fig. 2.

$Fig. 2. Expression of FLT-4 and FLK-1 in TrHBMEC. TCL (1 mg) from TrHBMEC were immunoprecipitated with the anti–FLK-1 antibody or FLT-4 antibody. Immunoprecipitates were size-fractionated on 7.5% SDS-PAGE, transferred onto a nitrocellulose membrane, and then immunoblotted with FLK-1 or FLT-4 antibodies. For FLK-1 expression, positive controls consisted of the same amounts of TCL from HUVEC. For FLT-4 expression, HEL-JW cells were used as a positive control. Normal rabbit serum and antibody alone were used as negative controls.$

Expression of FLT-4 and FLK-1 in TrHBMEC. TCL (1 mg) from TrHBMEC were immunoprecipitated with the anti–FLK-1 antibody or FLT-4 antibody. Immunoprecipitates were size-fractionated on 7.5% SDS-PAGE, transferred onto a nitrocellulose membrane, and then immunoblotted with FLK-1 or FLT-4 antibodies. For FLK-1 expression, positive controls consisted of the same amounts of TCL from HUVEC. For FLT-4 expression, HEL-JW cells were used as a positive control. Normal rabbit serum and antibody alone were used as negative controls.

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