Western blot analyses of G289 MoAb clones. Total cell lysates from the E2A/pbx1 carrying line 697 (A) and the Burkitt's lymphoma cell line Namalwa (B) were resolved on 4% to 20% SDS-PAGE and transferred to Immobilon-P membrane. Individual membrane strips were probed with various G289 clones, an E2A-specific G193-86 clone, and an isotype control MoAb using enhanced chemoluminescence.