Fig. 1.
Fig. 1. Analysis of αIIbβ3 expression (A), DNA content (B), and morphology (C and D) of CD34+ cells, performed before or after 6 days of liquid culture with 100 ng/mL TPO. αIIbβ3 expression was examined with anti-CD41a MoAb directly conjugated to fluorescein and fluorescence was analyzed by FACScan. Negative controls are represented by cells treated with an isotype-matched irrelevant MoAb (anti-CD8) directly conjugated to fluorescein. The DNA content was examined by FACScan after PI staining. Cytospins were observed at light microscopy after May-Grünwald Giemsa staining (original magnification × 400). Flow cytometry results are expressed in arbitrary fluorescence units and represent means ± SD of four separate experiments.

Analysis of αIIbβ3 expression (A), DNA content (B), and morphology (C and D) of CD34+ cells, performed before or after 6 days of liquid culture with 100 ng/mL TPO. αIIbβ3 expression was examined with anti-CD41a MoAb directly conjugated to fluorescein and fluorescence was analyzed by FACScan. Negative controls are represented by cells treated with an isotype-matched irrelevant MoAb (anti-CD8) directly conjugated to fluorescein. The DNA content was examined by FACScan after PI staining. Cytospins were observed at light microscopy after May-Grünwald Giemsa staining (original magnification × 400). Flow cytometry results are expressed in arbitrary fluorescence units and represent means ± SD of four separate experiments.

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