Fig. 3.
Progression of cytokine-stimulated Lin− Rholow/Holow cells through G0 /G1 and into S phase. Lin− Rholow/Holow were incubated in medium containing a cocktail of either 4 cytokines (IL-3, IL-6, IL-11, and SCF; A) or 7 cytokines (IL-3, IL-1α, GM-CSF, G-CSF, SCF, FGF, and CSF-1; B), and at indicated intervals duplicate aliquots of cells were analyzed for 3H-thymidine incorporation followed by autoradiography to assess the percentage of cells with labeled nuclei as described in the Materials and Methods. Each set of symbols in (A) represents observations from separate individual experiments.