Fig. 6.
Progression of cytokine-stimulated Lin−/Sca+ cells through G1 and into S phase of cell cycle. Lin−/Sca+ cells were incubated in medium containing SCF alone, SCF plus IL-3, or a cocktail of 7 cytokines (IL-3, IL-1α, GM-CSF, G-CSF, SCF, FGF, and CSF-1), and at indicated intervals duplicate aliquots of cells from each group were analyzed for 3H-thymidine incorporation followed by autoradiography to assess the percentage of cells with labeled nuclei as described in the Materials and Methods.