TF-1 cells adhere to fibronectin via integrin. Radiolabeled TF-1 cells suspended in RPMI 1640 medium containing 1% BSA (RPMI-BSA medium) or RPMI-BSA medium containing RGDS peptide (1 mg/mL), RGES peptide (1 mg/mL), antiintegrin antibodies (50 μg/mL each), or control isotype-matched IgG (50 μg/mL) were plated onto tissue culture plates (96-well, 1 × 10⁵ cells/well) coated with fibronectin (20 μg/mL). Cells were incubated for 20 minutes at 37°C. Cell adhesion assay was done as described in Materials and Methods. The values represent the mean ± standard error (SE) of triplicate assays for one representative experiment of three independent experiments.