Fig. 2.
Fig. 2. Effects of SLF on the adherence of TF-1 cells to fibronectin. (A) Dose-dependent increase of adhesion induced by SLF. TF-1 cells were plated on the fibronectin-coated wells in the presence of increasing amounts of SLF and were incubated for 30 minutes at 37°C. Cell adhesion assay was done as described in Materials and Methods. The values represent the mean ± SE of triplicate assays for one representative experiment of three independent experiments. (B) Inhibition of SLF-induced adhesion to fibronectin by wortmannin. TF-1 cells were preincubated with increasing concentrations of wortmannin for 30 minutes at 37°C, the cells were then plated on the fibronectin-coated wells in the presence of SLF (10 ng/mL). Cell adhesion assay was done as described in Materials and Methods. The values represent the mean ± SE of triplicate assays for one experiment.

Effects of SLF on the adherence of TF-1 cells to fibronectin. (A) Dose-dependent increase of adhesion induced by SLF. TF-1 cells were plated on the fibronectin-coated wells in the presence of increasing amounts of SLF and were incubated for 30 minutes at 37°C. Cell adhesion assay was done as described in Materials and Methods. The values represent the mean ± SE of triplicate assays for one representative experiment of three independent experiments. (B) Inhibition of SLF-induced adhesion to fibronectin by wortmannin. TF-1 cells were preincubated with increasing concentrations of wortmannin for 30 minutes at 37°C, the cells were then plated on the fibronectin-coated wells in the presence of SLF (10 ng/mL). Cell adhesion assay was done as described in Materials and Methods. The values represent the mean ± SE of triplicate assays for one experiment.

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