American Society of Hematology

Fig. 2.

$Fig. 2. Long-term lymphohematopoietic reconstitution by bcl-2–deficient mouse BM stem cells (A) and fetal liver cells (B). Transplantation was performed as described in experimental procedures. Peripheral blood mononuclear cells from the recipient mice transplanted with bcl-2−/− (closed squares and circles) or +/− (open squares) mouse-derived HSC were analyzed at each experimental period. The cells were stained with donor type common leukocyte antigen specific MoAb (anti-Ly5.2), recipient specific (anti-Ly5.1) MoAb, Thy-1/B220 mixture and Gr-1/Mac-1 mixture. Four-color flow cytometry analysis was performed. The percentage of Thy-1/B220 positive cells or Gr-1/Mac-1 positive cells in donor-derived cells (Ly5.2 positive fraction) were calculated. Kinetics of donor derived cells (left), and the relative percentage of donor derived lymphoid cells (middle), and myeloid cells (right) are shown. Each data point represents mean ± SD from 16 individual experiments.$

Long-term lymphohematopoietic reconstitution by bcl-2–deficient mouse BM stem cells (A) and fetal liver cells (B). Transplantation was performed as described in experimental procedures. Peripheral blood mononuclear cells from the recipient mice transplanted with bcl-2−/− (closed squares and circles) or +/− (open squares) mouse-derived HSC were analyzed at each experimental period. The cells were stained with donor type common leukocyte antigen specific MoAb (anti-Ly5.2), recipient specific (anti-Ly5.1) MoAb, Thy-1/B220 mixture and Gr-1/Mac-1 mixture. Four-color flow cytometry analysis was performed. The percentage of Thy-1/B220 positive cells or Gr-1/Mac-1 positive cells in donor-derived cells (Ly5.2 positive fraction) were calculated. Kinetics of donor derived cells (left), and the relative percentage of donor derived lymphoid cells (middle), and myeloid cells (right) are shown. Each data point represents mean ± SD from 16 individual experiments.

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