Fig. 1.
Fig. 1. TPT treatment results in selective depletion of the Mr ∼100,000 topo I signal. (A through E) HL-60 cells were treated with DMSO (lanes 1 through 4 and 9) or 100 μmol/L (lane 5), 30 μmol/L (lane 6), 10 μmol/L (lane 7), or 3 μmol/L (lane 8) TPT for 45 minutes, lysed under denaturing conditions, and prepared for SDS-polyacrylamide gel electrophoresis. Gels were loaded with polypeptides from 3 × 105 (lanes 1 and 5 through 9), 1.5 × 105 (lane 2), 0.75 × 105 (lane 3), or 0.3 × 105 cells (lane 4). After electrophoresis, samples were transferred to nitrocellulose. Blots were probed with antibodies that recognize topo I (A), topo IIα (B), poly(ADP-ribose) polymerase (C), B23 (D), or histone H1 (E). (F ) Relative topo I signal (from [A]) plotted as a function of TPT concentration.

TPT treatment results in selective depletion of the Mr ∼100,000 topo I signal. (A through E) HL-60 cells were treated with DMSO (lanes 1 through 4 and 9) or 100 μmol/L (lane 5), 30 μmol/L (lane 6), 10 μmol/L (lane 7), or 3 μmol/L (lane 8) TPT for 45 minutes, lysed under denaturing conditions, and prepared for SDS-polyacrylamide gel electrophoresis. Gels were loaded with polypeptides from 3 × 105 (lanes 1 and 5 through 9), 1.5 × 105 (lane 2), 0.75 × 105 (lane 3), or 0.3 × 105 cells (lane 4). After electrophoresis, samples were transferred to nitrocellulose. Blots were probed with antibodies that recognize topo I (A), topo IIα (B), poly(ADP-ribose) polymerase (C), B23 (D), or histone H1 (E). (F ) Relative topo I signal (from [A]) plotted as a function of TPT concentration.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal