Fig. 1.
![Fig. 1. Activation of HPg by lmw-uPA. wtr-E1-HPg (100 nmol/L) was activated by lmw-uPA (0.5 nmol/L) in the presence of the chromogenic substrate, S2251 (0.6 mmol/L). The HPm generated as a function of time was detected by the liberation of p-nitroanilide from the substrate and is depicted here as the absorbance at 405 nm. The curves illustrated are for wtr-E1-HPg and for the mutant, r-[R561A]E1-HPg. The reduced DodSO4/PAGE gel insert shows the protein components present after 10 minutes of activation. The buffer was 10 mmol/L HEPES-NaOH/150 mmol/L NaOAc, pH 7.4, at 37°C.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/5/10.1182_blood.v89.5.1585/3/bl_0001f1.jpeg?Expires=1735564679&Signature=BVMJ0Ngfu5RCwez81cFxF58Ta2yg8VfgMEF4upqWCH5AdU7V7dXQJoWR-uP0bEkOdIKUVsovW9eHmEKcEMwK-YSKUx2basNUZX1z70ilIWTBV4QNe0PF0KehfoqsKS6YbfUw42OGzzQQflZyEzhJhvmcXqyMT2FJJl~4KNFpyX7wy~YRkYphwmNNS7dTv95FQjrYS7BLmgF1j5c1BmFhavAl4aZgN4VyzIJFD7SyE5s0W2iCMbaP6FYOhAS4ml8RHvf4kYYj9rboM4WdGCBmuCeZ0aLhrhWM9gpPCNg93ayZSCnLlXsDeNmTJPuhRu69iVQCfhJzoTKHuwxB66XebQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of HPg by lmw-uPA. wtr-E1-HPg (100 nmol/L) was activated by lmw-uPA (0.5 nmol/L) in the presence of the chromogenic substrate, S2251 (0.6 mmol/L). The HPm generated as a function of time was detected by the liberation of p-nitroanilide from the substrate and is depicted here as the absorbance at 405 nm. The curves illustrated are for wtr-E1-HPg and for the mutant, r-[R561A]E1-HPg. The reduced DodSO4/PAGE gel insert shows the protein components present after 10 minutes of activation. The buffer was 10 mmol/L HEPES-NaOH/150 mmol/L NaOAc, pH 7.4, at 37°C.
