Fig. 2.
![Fig. 2. Activation of HPg by SK. wtr-E1-HPg (10 nmol/L) was activated by SK (10 nmol/L) in the presence of the chromogenic substrate, S2251 (0.9 mmol/L). The HPm generated as a function of time was detected by the liberation of p-nitroanilide from the substrate and is depicted here as the absorbance at 405 nm. The curves illustrated are for wtr-E1-HPg and for the mutant, r-[R561A]E1-HPg. The reduced DodSO4/PAGE gel insert shows the protein components present after 30 minutes of activation. The buffer was 10 mmol/L HEPES-NaOH, pH 7.4, at 37°C.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/5/10.1182_blood.v89.5.1585/3/bl_0001f2.jpeg?Expires=1735565968&Signature=b2sxSiG59vCa2McFl-iYewW49n~EecV0hnd2CzxOEK8hpgT0nsxujnUTsit4iDk7aa90tJ-ck3F4pvYtc5jKSBcwrPkndvcMgU0BVwoUw6XdpiktpG~tpI3t2f5PsHWN06suGMNb2iyIqf1-JCnmJGiQWrixkYImV1SDEaDBEhlKj76e4cbaq4YRlQZBxLhV5fVkxi6eHeP2DUJ5fnvxkDISKZx6PwGgSUj7Ig1CqIcah187cWaPzRAJllf-E4W-Gt99zyt6zvPzTYv1Ez2XPK4EXqIHFnL2GaTrqelK0kcWmUwC3g0Nm1oDHL9optEaYzgtMTtwJH-wYdPibtgdDQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of HPg by SK. wtr-E1-HPg (10 nmol/L) was activated by SK (10 nmol/L) in the presence of the chromogenic substrate, S2251 (0.9 mmol/L). The HPm generated as a function of time was detected by the liberation of p-nitroanilide from the substrate and is depicted here as the absorbance at 405 nm. The curves illustrated are for wtr-E1-HPg and for the mutant, r-[R561A]E1-HPg. The reduced DodSO4/PAGE gel insert shows the protein components present after 30 minutes of activation. The buffer was 10 mmol/L HEPES-NaOH, pH 7.4, at 37°C.
