Fig. 4.
![Fig. 4. Identification of mi protein in mast cells. MC-9 cells were labeled with [35S]-methionine. mi was identified by preincubating the antibody with or without the mi peptide before the addition of the antibody to the cell lysates (A). Immunoprecipitates of [35S]-methionine–labeled mi from either MC-9, spleen, or liver cells are shown in (B). Immunoprecipitates of [35S]-methionine–labeled mi from either BMMC or BM cells are shown in (C). Immunoprecipitates were analyzed by SDS-PAGE. One representative experiment of two performed is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2999/4/bl_0007f4b.jpeg?Expires=1735508413&Signature=vYl5Lv1P494R4hQkGVh~jen3UfVVEf94wj5oWP66tEvajUVlsAIc3n7VWHm94rFmnwkiwwHiS47DqYA~3fodcidEp4975jcSEYpqMf4de3u75fQQ6ZW6ms~zP8WqNqcuhbYco9o2N5bNZ2obHHHgp8A72ZBELXyHMnplkjzJ7kT62ohuRCkpDf6yyRGbOFPe8i2AgdNB2jTpMpferdfi-T0iqw5af~ZZQU1HsPPS2AAuhV9GpCisVUBcuFoi2SluL4uSeO-DJYOnrYNP4qqc~k9VGyQRD~grJO4kr1dW7BnfZAAGiWWD00N71ihcRV7CPI5Mnqnl~LMmyHJ29buYkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Identification of mi protein in mast cells. MC-9 cells were labeled with [35S]-methionine. mi was identified by preincubating the antibody with or without the mi peptide before the addition of the antibody to the cell lysates (A). Immunoprecipitates of [35S]-methionine–labeled mi from either MC-9, spleen, or liver cells are shown in (B). Immunoprecipitates of [35S]-methionine–labeled mi from either BMMC or BM cells are shown in (C). Immunoprecipitates were analyzed by SDS-PAGE. One representative experiment of two performed is shown.
