Fig. 7.
![Fig. 7. Association of mi with USF2 in activated MC-9 cells. [35S]-methionine–labeled activated cells (IL-3, IgE-DNP, or IL-4) were lysed with RIPA and incubated with protein A-bound anti-mi antibody. Immunocomplexes were then dissociated from the beads and either reprecipitated or not on protein A bound USF2 (A). Alternatively, [35S]-methionine–labeled IL-3–activated MC-9 cells were lysed with RIPA and incubated with protein A-bound anti-USF2 antibody. The immunocomplexes were then dissociated from the beads, diluted, and reprecipitated on either protein A-bound mi (B) or myosin (C) antibodies. Analysis was by SDS-PAGE. One representative experiment of three performed is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/8/10.1182_blood.v89.8.2999/4/bl_0007f7c.jpeg?Expires=1735509072&Signature=CcEEJ0P0S4Xzy45pMN-myefjY-8tsD3np4SUCddHCDkI5rQezTldXNcAAHreeiqivPcWfK4dFwDJdmFWDMF3XOwdkDQVZ~mWKX~Amov5mNYPIQI2YO15TQlpOGKA7j~MHE~AaDWZw1KioaGI0Ac~LBgxsu1ug-RbaRE5pKCYL1N0F4wHD6Fcg~hAVod1cENCcAnYanl7ENVbFsmKrFHoMA0DJhupUMLAw~8y7J0Ok-fCfpOuiO0xOVYPUq4i05tXVPvEN5GWRw4cPv5o-aevLJDk33oR7vP9bg8YBxazc-LazV2HK782Igb7ucN3rmk4y002jK-Y8Ae1UQIaBOcVEg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Association of mi with USF2 in activated MC-9 cells. [35S]-methionine–labeled activated cells (IL-3, IgE-DNP, or IL-4) were lysed with RIPA and incubated with protein A-bound anti-mi antibody. Immunocomplexes were then dissociated from the beads and either reprecipitated or not on protein A bound USF2 (A). Alternatively, [35S]-methionine–labeled IL-3–activated MC-9 cells were lysed with RIPA and incubated with protein A-bound anti-USF2 antibody. The immunocomplexes were then dissociated from the beads, diluted, and reprecipitated on either protein A-bound mi (B) or myosin (C) antibodies. Analysis was by SDS-PAGE. One representative experiment of three performed is shown.
