Fig. 6.
Neutrophil adhesion to MoAb-captured Lec-EGF under static conditions. Microtiter wells were coated with the MoAb 1478. Lec-EGF was captured in the indicated wells at a density of 2,780 sites/μm2. Neutrophils preincubated in the presence or absence of the anti–PSGL-1 MoAbs PL1 or PL2 were then added to the wells. Adhesion under static conditions was measured as described in the Materials and Methods. The data represent the mean ± SD of triplicate determinations. Similar results were obtained in two other experiments.