Neutrophil adhesion to MoAb-captured Lec-EGF or sPS under hydrodynamic flow conditions. Lec-EGF or sPS was captured at the indicated density on 35-mm dishes with immobilized MoAb 1478 or S12, respectively. As a control, one dish was blocked with HSA but was not incubated with MoAb or selectin. Each dish was inserted into a parallel-plate flow chamber. (A) Neutrophils were perfused through the chamber at a wall shear stress of 1 dyn/cm². After 4 minutes, the number of adherent cells (all of which rolled on the surface) were quantified. (B) The velocities of 50 individual neutrophils rolling on either Lec-EGF or sPS were measured. Similar results were obtained in two other experiments.