Fig. 1.
Proliferative response of unfractionated preG-PBMC and G-PBMC to allogeneic, DR-mismatched PBMC in unidirectional MLC. Different numbers (5.0, 2.5, and 1.25 × 104) of preG-PBMC (solid lines) or G-PBMC (dashed lines) responders were cultured with 1.0 × 105 irradiated (30 Gy) allogeneic, DR-mismatched PBMC stimulators in round-bottom 96-well plates. Values for 3H-thymidine incorporation represent the mean counts per minute (cpm) ± SEM from quadruplicate cultures with values from autologous background controls subtracted. Results from one of five representative experiments are shown.