Phenotypic analysis of preG- and G-CD4 and CD14 cells. PreG-PBMC (solid lines) and G-PBMC (dashed lines) from the same donor were stained with anti-CD4-PE or anti-CD14-PE plus FITC-conjugated antibodies against the indicated costimulatory molecules or HLA-DR. Samples were analyzed by gating on the CD4- or CD14-positive population, and fluorescence intensities were compared between preG- and G-CD4 and CD14 cells relative to isotype-matched control antibodies (thin lines). Results from one of three representative experiments are shown.