Release of (A) IL-6 and histamine and (B) [³H]-5HT and LTC₄ from BALB/c BMCMCs that had been stimulated with various concentrations of rrSCF. Release of IL-6, histamine, and LTC₄ was measured at 2 hours (from the same aliquots of cells), whereas [³H]-5HT release was measured at 10 minutes (in separate aliquots of the same cell preparations, but which had been incubated before challenge with [³H]-5HT; see the Materials and Methods). #P < .05 or *P < .001 versus values for unstimulated control cells. After sensitization with anti-DNP IgE and challenge with DNP30-40-HSA at 10 ng/mL (see the Materials and Methods), the same population of BMCMCs shown in (A) and (B) released 5,510 ± 270 pg IL-6 and 165 ± 10 ng LTC₄ per 10⁶ cells (at 2 hours) and gave 20% ± 2.3% specific release of histamine (at 2 hours) and 38% ± 0.7% specific release of [³H]-5HT (at 10 minutes). Data are shown as mean ± SEM (n = 4 to 5 per point) but, in many instances, error bars are too small to be seen in the figures.