Kinetics of IL-6 production, as assessed by measurements of (A) supernatant- or (B) cell-pellet-associated IL-6, or (C) total (supernatant-plus cell-pellet-associated) IL-6 in BALB/c BMCMCs after stimulation with rrSCF (250 ng/mL; ▪), LPS (250 μg/mL; ▧), IgE and DNP-HSA (10 ng/mL; ▧), or vehicle alone (□). *P < .001 versus corresponding values for vehicle-challenged cells (n = 3 to 5 per point). Please note that IL-6 associated with cell pellets from vehicle-challenged cells was below the limit of detection of the ELISA assay (ie, < 1 pg/10⁶ cells).